Changes

Preparing a SDS-PAGE Gel

137 bytes added, 16:38, 4 June 2020

added SOP requirement-JR

==SOP==

*[[SOP- Electrophoresis]]

==Separating Gel==

*For 12 mL final volume (2 gels)

| TEMED || 30 uL || 30 uL || 30 uL || 30uL

*For a [[Phos-Tag Electrophoresis and Western Blotting|Phos-Tag Gel]], add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl in the separating gel only

==Stacking Gel==

[[Category: SDS-PAGE]]

[[Category: Electrophoresis]]

[[Category: PhosTag]]

[[Category: Phosphorylation]]

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