164
edits
Changes
added SOP requirement-JR
==SOP==
*[[SOP- Electrophoresis]]
==Separating Gel==
*For 12 mL final volume (2 gels)
| TEMED || 30 uL || 30 uL || 30 uL || 30uL
|}
*For a [[Phos-Tag Electrophoresis and Western Blotting|Phos-Tag Gel]], add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnClin the separating gel only
==Stacking Gel==
| Water || 4 mL
|-
| APS (make fresh, 0.1g/mL in water) || 200 uL || 200 uL || 200 uL
|-
| TEMED || 30 uL || 30 uL || 30uL
|}
[[Category: SDS-PAGE]]
[[Category: Electrophoresis]]
[[Category: PhosTag]]
[[Category: Phosphorylation]]