Changes

Preparing a SDS-PAGE Gel

242 bytes added, 16:38, 4 June 2020
added SOP requirement-JR
==SOP==
*[[SOP- Electrophoresis]]
 
==Separating Gel==
*For 12 mL final volume (2 gels)
*Pour into cassette, overlay with the top layer of water saturated butanol
{| border="1"
! Component || 6% || 7.5% || 10% || 12.5%
|-
| [[SDS-PAGE Separating Gel Buffer]] || 3 mL || 4 3 mL || 5 3 mL || 3 mL
|-
| [[Acrylamide Solution]] || 3 2.4 mL || 3 mL || 3 4 mL || 5 mL
|-
| Water || 6.6 mL|| 6 mL || 5 mL || 4mL
|-
| APS (make fresh, 0.1g/mL in water) || 200uL || 200 uL || 200 uL || 200 uL
|-
| TEMED || 30 uL || 30 uL || 30 uL || 30uL
|}
*For a [[Phos-Tag Electrophoresis and Western Blotting|Phos-Tag Gel]], add 24 uL 5 mM Phos-Tag and 24 uL 10 mM MnCl in the separating gel only
==Stacking Gel==
| Water || 4 mL
|-
| APS (make fresh, 0.1g/mL in water) || 200 uL || 200 uL || 200 uL
|-
| TEMED || 30 uL || 30 uL || 30uL
|}
[[Category: SDS-PAGE]]
[[Category: Electrophoresis]]
[[Category: PhosTag]]
[[Category: Phosphorylation]]
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