Changes

Preparation of Tail Samples (for Genotyping)

No change in size, 15:20, 21 May 2018

changed g to ml for detergents

* 0.1 mg/mL MgCl2, 6H20

* 0.1 mg/mL Gelatin

* 0.45% (NP-40) Jgepal (Hint: Use cut tips, as NP-40 is very viscous)

* 0.45% Tween 20 (Hint: Use cut tips, as Tween 20 is very viscous)

~~PBDN~~ PBND can be made in liter volume, aliquoted into 50ml falcon tubes, frozen, and used as needed

For 250ml PBND:

* 0.930g 50mM KCl

* 0.300g 10mM Tris HCl (pH~8.3)

* 0.025g 0.1 mg/mL MgCl2, 6H20

* 0.025g 0.1 mg/mL Gelatin

* 1.~~125g~~ 125ml 0.45% (NP-40) Jgepal * 1.~~125g~~ 125ml 0.45% Tween 20

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edits