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Preparation of Tail Samples (for Genotyping)

5 bytes removed, 15:20, 21 May 2018
changed g to ml for detergents
* 0.1 mg/mL MgCl2, 6H20
* 0.1 mg/mL Gelatin
* 0.45% (NP-40) Jgepal (Hint: Use cut tips, as NP-40 is very viscous)
* 0.45% Tween 20 (Hint: Use cut tips, as Tween 20 is very viscous)
PBDN PBND can be made in liter volume, aliquoted into 50ml falcon tubes, frozen, and used as needed
For 250ml PBND:
 
* 0.930g 50mM KCl
* 0.300g 10mM Tris HCl (pH~8.3)
* 0.025g 0.1 mg/mL MgCl2, 6H20
* 0.025g 0.1 mg/mL Gelatin
* 1.125g 125ml 0.45% (NP-40) Jgepal * 1.125g 125ml 0.45% Tween 20 1. Start by adding 150ml ddH2O to a beaker on a stir plate (with magnetic stir bar).2. Add reagents. Mix well.3. Add 37% HCl drop-wise to adjust pH to 8.3.4. Add ddH20 to 250ml.
# Start by adding 150ml ddH2O to a beaker on a stir plate (with magnetic stir bar).
# Add reagents. Mix well.
# Add 37% HCl drop-wise to adjust pH to 8.3.
# Add ddH20 to 250ml.
==Protocol==
Reddj
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