164
edits
Changes
changed g to ml for detergents
* 0.1 mg/mL MgCl2, 6H20
* 0.1 mg/mL Gelatin
* 0.45% (NP-40) Jgepal (Hint: Use cut tips, as NP-40 is very viscous)
* 0.45% Tween 20 (Hint: Use cut tips, as Tween 20 is very viscous)
For 250ml PBND:
* 0.930g 50mM KCl
* 0.300g 10mM Tris HCl (pH~8.3)
* 0.025g 0.1 mg/mL MgCl2, 6H20
* 0.025g 0.1 mg/mL Gelatin
* 1.125g 125ml 0.45% (NP-40) Jgepal * 1.125g 125ml 0.45% Tween 20
==Protocol==
# Combine 100uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mLin ddH2O)
# Incubate at 55 degrees (16 hours - O/N)
# Incubate at 85 degrees for 60 min