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**Do NOT use a solution that is yellow or contains a precipitate.
**Avertin is lipid soluble and may require a larger dose.
==Perfusion Pump Set Up==
*Place 30 ½ gauge on end of tubing
*Insert one tube into 9% saline solution, and one tube into 10% formalin solution
''Image 1: Set up of the two tube system from 0.9% saline solution and 10% formalin solution.''
[[File:perfusionbottle.png]]
*Turn pump on to a flow rate of 0.72 ml/min
*Have stopcock turned to allow for flow of saline
[[File:perfusionstopcock.png]]
*Fill line with 0.9% saline until you have a steady stream of 0.9% saline from the end of the needle.
*Turn off peristaltic pump and turn stopcock to allow for formalin solution to flow
*Turn on peristaltic pump and pump 10% formalin solution until flow fluid is before the stopcock
**Flow has not entered the main line
*Turn off peristaltic pump and turn stopcock to allow for saline to flow
*Turn on pump and pump saline through the main line at stated flow rate
''Image 3: Overview of the entire perfusion setup (excluding the procedural platform in the collecting dish)''