Changes

PCR Analysis of Tail DNA

156 bytes added, 18:43, 17 February 2016
Protocol
==Protocol==
First, make 250ul of 0.4um working stock primers from 100um Primary Stocks.
#248 ul ddH20
#1ul forward primer (100um)
#1ul reverse primer (100um)
 
 
Use the following Volumes per 25ul Reaction:
Per sample (1X)
#Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
#0.4um Primer Mix: 5ul
#Sterile ddH2O: 7.5ul
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