Changes

'''Fixing adipogenic cultures'''

•Remove cells from incubator and place in the fume hood. All procedures involving formalin must be performed in a fume hood.

•Aspirate the media first off the control and then off the induced wells of the post-induction adipogenic plate. Note: Be sure not to let the cells remain dry for longer than 30 seconds at any point during the fixation or staining processes.

•Gently rinse the plate with 2 ml of sterile DPBS along the sides of each well of the adipogenic plate so as not to disturb the monolayer.

•Aspirate the DPBS and add 2 ml of 10% formalin along the sides of each well of the plate, again being careful not to disrupt the cells.