Open main menu

Changes

Mutagenesis

No change in size, 00:29, 2 May 2009
→‎Protocol
==Protocol==
#Mix:
**##5 uL 10X Reaction Buffer**##~20 ng Template (1 uL of minprep)**##10 uL of Primer Mix**##5 uL of dNTP mix**##28 uL of water
#Add 1 uL of Pfu Ultra HF Polymerase
#Run PCR Program (DAVE-MUT)