Measuring Fasting Insulin

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Materials

  • Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
  • Heparinzed capilaries

Protocol

  1. Collect about 2/3 of a capillary of blood.
  2. Transfer blood to labelled eppendorf tubes.
  3. Let blood clot
  4. Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge)
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