Difference between revisions of "Main Page"
From Bridges Lab Protocols
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*[[TOPO Cloning]] | *[[TOPO Cloning]] | ||
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==Protein Analysis== | ==Protein Analysis== | ||
Revision as of 14:22, 27 May 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Protein Analysis
Protein Quantification
- Bradford Assay
- Quantification by Absorbance at 280nm
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
