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Lipofectamine Plasmid Transfection

28 bytes added, 14:45, 13 August 2009
m
→‎Protocol (6 well dish)
==Protocol (6 well dish)==
#Plate cells the day before so that they are at 90-95% confluence in 2 mL media without antibiotics(DMEM with 10% FBS, no PSG).
#For each well prepare:
##250uL OptiMEM plus 4 ug of DNA.