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Lipofectamine Plasmid Transfection

61 bytes removed, 18:18, 12 August 2009
m
→‎Protocol (6 well dish)
==Protocol (6 well dish)==
#Plate cells the day before so that they are at 90-95% confluence in 2 mL media without antibiotics, or alternatively change media before transfection to OptiMEM.
#For each well prepare:
##250uL OptiMEM plus 4 ug of DNA.