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Lipofectamine Plasmid Transfection

65 bytes added, 21:00, 11 July 2012
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#Add DNA/Lipofectamine complexes (~500 uL) to the 2 mL of media on the cells
#Gently rock plate to mix
#After ~4h aspirate media and re-feed cells with normal media. (It is usually possible to leave overnight).
Protocol adapted from [http://tools.invitrogen.com/content/sfs/manuals/lipofectamine2000_man.pdf Product Manual]
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