Changes

Inositol Labeling and Lipid Extraction

21 bytes removed, 15:41, 7 August 2009
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Materials
==Materials==
*Cells in 100 mm culture dishes*Labelling Media (Inositol Free DMEM with PSG, 10% dialysed serum and 100 uCi inositol per plate
of cells)
*Perchloric acid (640 uL into 10 mL water) keep cold*100 mM EDTA (2 mL of 0.5M stock in 10 mL) keep cold*Water*Deacylation Mix (3.5 mL Methylamine, 6 mL Methanol, 1.5 mL Butanol, 2.1 mL Water)*Lipid Extraction Mix (10 mL Butanol, 2 mL Ethyl Ether and 0.5 mL Ethyl Formate)
==Protocol==