Changes

H&E Staining of Mouse Tissue

30 bytes added, 14:51, 9 June 2015

→‎Protocol

==Materials==

*xylene/Citrisolv

*100% Ethanol

*70% Ethanol

# Place slide on edge and bake for 15 min at 60 degree C to melt wax

# Deparaffinize and rehydrate sections

## Immerse for 5 min with agitation in xylene/Citrisolv

## Immerse for 5 min with agitation in 100% ethanol

## Immerse for 5 min with agitation in 70% ethanol

#Place slides in 95% Ethanol two times for 30 seconds each.

#Transfer slides into 100% Ethanol for 30 seconds, repeat with fresh 100% Ethanol.

#Wash twice with fresh xylene /Citrisolv for 30 seconds.

#Let slide dry.

#Place 1-2 drops of mounting medium on slide and place coverslip over

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