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H&E Staining of Mouse Tissue

156 bytes added, 16:16, 27 March 2015
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##depending on the intensity desired, repeat this step if necessary
#Dip slide in 1% eosin Y fo 10-30 sec with agitation
*Dehydration
#Place slides in 95% Ethanol two times for 30 seconds each.
#Transfer slides into 100% Ethanol for 30 seconds, repeat with fresh 100% Ethanol.
#Wash twice with fresh xylene for 30 seconds.
#Let slide dry.
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