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Glycogen Determination from Tissues

6 bytes added, 18:21, 18 May 2012
m
bolded vial type
==Protocol==
# Weight out 30-90 mg tissue into *a '''screw cap vial* ''' and record weights. Screw cap vials are really important or else the lids will pop off
# Add 300 uL 30% KOH and place on 95C heat block for 30 min with occasional mixing.
# Cool and then add 100 uL Sodium Sulfate and 800 uL Ethanol.