Changes

Glucose Uptake Assay

17 bytes added, 00:13, 27 May 2009
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#After 5 min add 50 uL cold 2-DG
#Wash cells 3x1mL with cold PBS
#Add 500 uL PBS per well#Scrape cells with a an upside down p200 tip
#Add 400 uL cells to 5 mL aqueous scintillation fluid, vortex and count on program 3
#Do a bradford assay on 20 uL of cells (use PBS as blank)