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FM4-64 Labeling of Yeast Cells

3 bytes removed, 15:24, 27 January 2010
updated synaptored concentration
==Materials==
*Instead of FM4-64 we use SynaptoRed (Sigma #S6689). This is resuspended 1mg 5mg to 500 514 uL (2ug/mL16mM) with DMSO and stored in '''Molecular Biology Stuff'''
*Media (see [[YPD Media and Agar]])
*Dye solution. For each cell line need 190 200 uL media + 60 1 uL dye.
==Protocol==