Dilution Formula

C1V1=C2V2

  • C1=measured absorption
  • V1=unknown volume to be added to final volume
  • V2=desired volume to re-suspend V1 in (usually 4mL)
  • C2=OD/2^x
  • x=amount of times cells will double
  • find this by dividing the number of hours the cells will be growing by how long one doubling period is