Deparaffinizing Histology Slides

Materials

1. Xylene
2. 100% Ethanol
3. 95% Ethanol

Method

• Use slide rack/holder

1. Xylene: 2 x 3 minutes
2. Xylene 1:1 with 100% Ethanol: 3 minutes
3. 100% Ethanol: 2 x 3 minutes
4. 95% Ethanol: 3 minutes
5. 70% Ethanol: 3 minutes
6. 50% Ethanol: 3 minutes
7. Running cold tap water to rinse

Keep the slides in the tap water until ready to perform staining protocol.

https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf