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Collecting and Storing Mouse Serum

Revision as of 15:54, 18 January 2024 by Davebridges (Talk | contribs) (created serum page)

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Revision as of 15:54, 18 January 2024 by Davebridges (Talk | contribs) (created serum page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Materials

  • Heparinzed capilaries (Fisher cat# 22-362574)

Protocol

  1. Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
  2. Transfer blood to labelled eppendorf tubes.
  3. Let blood clot for 30 min on ice.
  4. Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).
  5. Gentry remove liquid layer and store at -80
Last modified on 18 January 2024, at 15:54