162
edits
Changes
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
==== Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads====
# Add 20 μl re-suspended magnetic bead slurry (for each sample you plan to use with this antibody) to a 1.5 ml microfuge tube on ice containing 1 ml cold PBS/BSA. Mix well, '''do not vortex'''. ''Note: For each wash , you will want to invert the tube while on the magnet rack to collect the beads that remain in the liquid at the top of the tube. This will allow you aspirate the liquid from the top of the tube between washes without sucking up any of the beads.''
# Place the microfuge tubes on the magnet rack and remove supernatants.
# Resuspend the beads in 1 ml cold PBS/BSA.