162
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Changes
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
==== Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads====
# Add 20 μl re-suspended magnetic bead slurry (for each sample you plan to use with this antibody) to a 1.5 ml microfuge tube on ice containing 1 ml cold PBS/BSA. Vortex briefly to mix Mix well, '''do not vortex'''.
# Place the microfuge tubes on the magnet rack and remove supernatants.
# Resuspend the beads in 1 ml cold PBS/BSA.