162
edits
Changes
→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
** [[High Salt Immune Complex Wash Buffer]] (Catalog # 20-155), one wash
** [[LiCl Immune Complex Wash Buffer]] (Catalog # 20-156), 3-5 washes
** [[TE Buffer]] (Catalog # 20-157), two washes"Note: for TE washes use a pipette to carefully aspirate, the beads seem to come off of the magnet easily with this wash"
=== Elution of Protein/DNA Complexes ===