Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
Materials
- 0.1M NaBicarbonate (0.8401g in 100mL ddH2O) and 0.5MNaCl (2.922g in 100mL ddH2O)at pH 8.0.
- 50mM Sodium Acetate (0.41g in 100mL ddH2O)at pH 4.0
- 50mM Tris (0.61g in 100mL ddH2O) and .5M NaCl (2.922g in 100mL ddH2O) at pH 8.0
- 0.4M glycine (3.004g in 100mL ddH2O)at pH 1.8.
Procedure Protein must be extensively dialysed in PPBS to remove any amine group from the sample.