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#Scrape cells and homogenize with dounce homogenizer for 20 strokes
#Centrifuge @ 3,000 rpm (800g) at 4 degrees for 10 minutes
#(Optional) Collect supernatant and spin @ 3,000 rpm at 4 degrees for 5 minutes
#Collect supernatant and spin in TLA 100.3 rotor at 19,000 rpm for 20 minutes at 4 degress (Tube = Beckman #349622)
#(Optional) Wash pellet in 1.0 mL HES and spin in TLA 100.3 rotor at 19,000 rpm for 20 minutes at 4 degress (Tube = Beckman #349622)#(Optional) To further purify PM, repeat steps #4 and #6
#Resuspend pellet in 1.0 mL HES and homogenize with dounce for 10 strokes
#Layer the resuspended pellet on top of 10 mL 40% sucrose/HES in a 13.5 mL ultracentrifuge tube (tube = Beckman #331372)