Changes

3T3-L1 Adipocyte Fractionation

8 bytes removed, 12:40, 4 May 2009
Optiprep Gradient
#Centrifuge 4h at 62 000 RPM in NVT90 at 4C.
#Cut off top of tube and gently remove 350 uL fractions to fresh tubes on ice.
#Store samples at -80. Make up SDS samples and <bold>'''do not boil</bold>'''
==References==
<pubmed>17765682</pubmed>