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CHAPS Lysis Buffer

524 bytes added, 15:24, 15 July 2009
Created page with '==CHAPS Lysis Buffer (for 10mL lysis buffer)== {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 40 mM || 400 uL || 1M || 4C |- | NaC...'
==CHAPS Lysis Buffer (for 10mL lysis buffer)==
{| border="1"
! || Final Concentration || per 10 mL || Stock || Location
|-
| HEPES, pH 7.4 || 40 mM || 400 uL || 1M || 4C
|-
| NaCl || 120 mM || 300 uL || 4M || Shelf
|-
| EDTA || 2 mM || 40 uL || 0.5M || Shelf
|-
| CHAPS || 0.3% || 300 uL || 10% ||
|-
| NaPPi || 10 mM || 400 uL || 0.25M || Shelf
|-
| beta-glycerophosphate || 10 mM || 100 uL || 1M || 4C
|-
| NaF || 50 mM || 1mL || 0.5M || Shelf
|-
| Protease Inhibitors || || 1 mini tablet || || 4C
|}
[[ Category:Buffer ]]

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