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b) Pre-prepared Schiff’s reagent - Australian Biostain Pty Ltd,PO Box 1407,Traralgon, VIC.
==Sigma-Aldrich Protocol==
*Using PAS Staining System: 395B-1KT
#Deparaffinze and hydrate sections to deionized water.
#Immerse slides in '''Periodic Acid Solution''' for 5 minutes at room temperature (18-26C).
#Rinse slide in several changes of distilled water.
#Immerse slides in '''Schiff's Reagent''' for 15 minutes at room temperature (18-26C).
#Wash slides in running tap water for 5 minutes.
#Counterstain slides in '''Hematoxylin Solution''', Gill No. 3, for 90 seconds.
#Rinse slides in running tap water.
#Dehydrate, clear, and mount sections in toluene or xylene based mounting media.
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/General_Information/2/395.pdf
