915
edits
Changes
m
Made section headers
__NOTOC__
[[ Category: ChIP ]]
[[ Category: RNA ]]
Johnson DS, Mortazavi A, Myers RM, Wold B. Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science (80- ) 316: 1497–1502, 2007. [http://dx.doi.org/10.1126/science.1141319 doi:10.1126/science.1141319]
==Before You Start==
===Buffers and Solutions Needed===
===Equipment===
* Cool microfuge and swinging bucket centrifuge down to 4C
==Protocol==
This protocol involves preparation of the crosslinked DNA, immunoprecipitation of the DNA and analysis by qPCR. It is possible to stop and freeze the samples after each of these steps.
===Crosslinking, Lysis and Shearing of DNA===
===Immunoprecipitation===
===Analysis of Immunoprecipitated DNA===