Changes

TaqMan SNP Assay

17 bytes added, 16:39, 16 November 2015

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# Dilute gDNA samples to 20ng/ul approx.

# Place gDNA, TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X) on ice.

# Make up Reaction Master mix (TaqMan® Genotyping Master Mix (40X), TaqMan® Genotyping Assay (2X): see table below).

# Add Reaction Mix (5.25 ul) added to each well (Table 1.)

# Diluted gDNA samples each added to a single well (Table 1.)

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