Changes

QPCR

78 bytes added, 22:12, 17 September 2014
added booking site
===Plate Preparation===
#Book 2h on qPCR machine by calling Felicia Waller (8-8746)going to http://slimprimplus.uthsc.edu/SlimPrim/index.php?action=workflow&module_id=4&sub=lc480logbookrequest
#Prepare cDNA and dilute in water in a 96 well plate. Typically a 20x dilution of cDNA leaves enough to be detected.
#Get optically clear 384 well plate and keep on paper towel. Do not touch bottom of plate.