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Ob/ob Genotyping

482 bytes added, 19:59, 26 September 2013
added nots on how to make primer mixes and added band sizes
* ob/ob - WT-Lep-F: 5'- AAT GAC CTG GAG AAT CTC C -3'
* ob/ob - Lepob-R: 5'- GCA GAT GGA GGA GGT CTC A -3'
 
===Primer Mixes===
Primers are resuspended at 100 uM, so add 3x10 uL of the primers to 970uL of water to make the primer mixes:
*For '''WT Primers''' combine both RFLP primers with the WT-Lep-F primer
*For '''Ob Primers''' combine both RFLP primers with the Lebob-R primer
==PCR Reaction==
==Bands==
[[File:ob_ob.jpg]]There should be a control band in all lanes at 191bpFor the wt primers if there is one or more wt alleles there should be a band at 104bpFor the ob primers, if there is one or more ob alleles there should be a band at 123bp 
[[ Category:Genotyping ]]