34
edits
Changes
m
→Protocol: Added Protein Lysate
==Protocol==
Cuvette Bradford Assay
#Dilute reagent 5X in water, stable for 2-3 weeks
#Pipet 1 mL into disposable plastic cuvette
##Blank then measure samples, absorbance must be less than 0.9
##Print (hit Recall, then enter, then print) and attach to experiment
Protein Lysate Bradford Assay
#Dilute reagent 5X in water, stable for 2-3 weeks
#In a 96 well plate, dilute sample 20X (190ul Reagent, 10ul Sample)
#Add 5ul of sample to 100ul of reagent in well
#Run "Bradford Assay Protocol" on Plate Reader
==Reference==
*Wikipedia: [[wikipedia:Bradford_protein_assay|Bradford Protein Assay]]
*PMID 942051