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→Protocol
#Template: 1 uL
Master Mix(Per 5mL -- Make 1mL Aliquots)#10X GoTaq Buffer: 50uL 625uL ("Molecular Biology Stuff" box in freezer) #Primer Mix: 50ul625ul#dNTPs: 62.5uL of 10 mM ("Molecular Biology Stuff" box in freezer)#Sterile water: 290ul 3625ul #Polymerase Go-Taq: 115.25ul 625ul ("Molecular Biology Stuff" box in freezer)
*Add Template Individually