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==Preparing the Crosses==
*Collect virgin females with appropriate marker and put into a new vial.
*Store the vials from which virgins are collected at 18° C when not using.
*Once 10 virgin females from a strain have been collected, add 4 males with the appropriate marker from a different strain.
*Label the vial to indicate the cross type and generation (A1 = the first generation of the first cross).
*Store the crosses in an appropriate temperature-controlled incubator.
*After 5 days, flip the 14 flies into a new vial (there should be eggs visible at the surface of the food).
*Label the new vial appropriately (A2 = the second generation of the first cross).*Repeat this cycle until enough progeny from the crosses have been produced or until there are no parent males left. ==Collecting the Progeny==* The progeny from the crosses will start eclosing after ~10 days.
* Record the date that the flies begin eclosing.
* Once there are at least 20 flies in the vial, collect the progeny and sort according to phenotype.
* Place the males and females into separate vials.
* There should be 5 - 15 progeny flies in each vial.
**If necessary, separate/combine phenotypes (but not gender) to have an appropriate number of flies in the vial.
* Label the vial with:
#Cross type/generation
#date born
#date added to vial.
* Keep the progeny vials in an appropriate incubator.* Repeat this process during the six days following the date of first eclosure.**Wait to sort until there are at least 20 flies in the vial (usually 2 to 3 times per vial).**Record the most likely date of birth before each sorting. *After six days, toss the vial. ==Recording Deaths of the Progeny==* Flip all progeny vials into fresh vials twice a week(every 3-4 days).*Record information on any dead flies found while flipping:#Cross type#Gender#Phenotype#Date born#Date of death