915
edits
Changes
added ason's papers
* [http://pubmed.org/17921418 Optimization of dietary restriction protocols in Drosophila] J. Gerontol
* [http://10.1371/journal.pone.0004067 Effect of a standardised dietary restriction protocol on multiple laboratory strains of Drosophila melanogaster] PLOS One
From Ason Chiang
I just find the method for measuring life span.
http://www.pnas.org/content/102/8/3105.full.pdf+html
http://www.pnas.org/content/102/8/3105/suppl/DC1#F1
that is described as follow:
Lifespan. UAS-rpr/+; dilp2-GAL/+, dilp2-GAL/+, UAS-rpr/+ and wDah flies were reared at standard larval density. Eclosing adults were collected over a period of 8 hours. Lifespan was measured in flies (10 flies per vial) on standard food medium, transferred to new food twice weekly by CO2 anaesthesia, and randomly sorted to maintain a density of 10 flies per vial. For mortality analysis, an otherwise similar experiment was done with flies maintained at a density of 100 files per bottle on standard food. Deaths were scored five or six times every 7 days.
I think the method looks reasonable. What do you think?
also you might be interested in this review.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3087113/