915
edits
Changes
added rapamycin treatment from Lamming et al
*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
*Inject 10 uL/g body weight
==Alternate Rapamycin Injection Regimen==
* Phenotypes seem to be different with rapamycin as treated by Lamming et al.
* This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135
<blockquote>
rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally
once daily with either 2 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a
concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.
</blockquote>