915
edits
Changes
removed confusing buffer addition
#Heat shock at 42C for 45s
#Place back on ice
#Add 450 uL of SOC Buffer or LB on shelf OR add 1000uL and adjust the level.
#Incubate at 37C for 1h with occasional mixing
#Plate 30-50 uL for amplification, or all for cloning/mutagenesis
*When plating be sure to label plates and use extreme caution when sterilizing the spreader with EtOH and fire.