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rewrote procedure
# Turn radioactive tissue culture incubator on at 37C, make sure to turn on the CO2 as well.
# Starve cells in '''Low Glucose Starvation Media''' for >3h.
# Prepare '''Acetate Incorporation Media''' Make at least enough for one extra well, using 0.5 mL50 uL/well for a 12 well dish. If monitoring lipogenesis in a line other than adipocytes, increase the hot glucose to 50 uCi per mL.
# Pretreat cells with inhibitors if required.
# Change Media to '''Acetate Incorporation Media''' and add Add 100 nM insulin as required.# Add 50 uL of Hot Acetate Solution to each well.# Place in the radioactive tissue culture incubatorfor 60-120 minutes.
# Save 3 x 5 uL of the '''Acetate Incorporation Media''' to count total radioactivity.
# After 60 min wash cells 3x with 1 mL of PBS.