Changes

Genotyping Program

497 bytes added, 15:06, 6 December 2010
Created page with '{| border="1" !| Allele || Program|| Fragment Size(s) || Notes |- |TSC-floxed || Inoki PCR || || Two bands if heterozygote |- |Cre || Inoki PCR || || Only present if Transgene...'
{| border="1"
!| Allele || Program|| Fragment Size(s) || Notes
|-
|TSC-floxed || Inoki PCR || || Two bands if heterozygote
|-
|Cre || Inoki PCR || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
|-
|Vac14 || Inoki PCR|| || Two bands if heterozygote
|-
|TC10b || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
|}
*Bring up to 500 mL with ddH20
*Sterile filter buffer and store at 4C
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]