Open main menu

Changes

Preparing an Adenoviral shRNA Clone

116 bytes added, 14:37, 14 September 2010
added protocol links
==Cloning Targetting Construct==
*see [http://www.oligoengine.com/products/psuper/documentation/protocols/pSUPER_protocol.pdfpSuper] and [http://www.coloncancer.org/adeasy/He,%20AdEasy%20-%20Nature%20Protocols,%202007.pdf AdEasy] protocols
===Digestion of pAdtrack-H1 Promoter (or pSuper)===
#If using a BglII/HindII do a sequential digestion