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Purification of GST-HA-S6K

82 bytes added, 15:32, 4 November 2009
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#Collect supernatant (save a lysate sample) into a 15 mL Falcon tube
#Add 0.5 mL Glutatione-sepharose beads and incubate end over end for 1-2h.
 
[[Category: Protein Purification]]
[[Category: mTOR]]
[[Category: Kinase Assay]]