Changes

#Warm PBS and Media in water bath

#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-

#Add 10 mL media to each new dish

#Check cells for trypsinization, and if necessary tap the cells

#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)

#Replace plates in the incubator

==Cell Specific Notes==

*3T3-L1 fibroblasts have special considerations regarding confluence. See [[Differentiation of 3T3-L1 Cells]]