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Glucose Uptake Assay

11 bytes added, 18:19, 14 October 2009
added link to KRBH Buffer
==Materials==
*2-Deoxyglucose (cold): Sigma D-6134. Dissolve 32.8mg/mL (200 mM) in KRBH
*0.5% BSA in [[KRBHBuffer]]
*Radioactive <sup>14</sup>C-2-deoxyglucose
*Hot 2-DG. Need 50 uL per well, prepare 1 mL KRBH/BSA, 1 uL cold 2-DG and 50 uL radioactive 2-DG