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==Materials==
*S2 Cells. See [[Culturing S2 Cells]]
*dsRNA. See [[Preparation of Designing and Preparing dsRNA]]*S2 Cell Media. See [[Culturing of S2 Cells]]
==Protocol==
**Count one row of 4 squares. For cells touching the edges of the square, only count the top and left line to avoid counting the same cells twice.
**Record the number of cells (should be 30-150 cells per row) and Calculate cell concentration, where x is number of cells per row
<pre>x * 4 (rows per square) * 10^5</pre>
**Dilute cells to 1^6 cells per mL and seed out in 0.5mL per 12 well.
**Let cells attach for >30 min, then add 0.5mL fresh media with 10 ug/mL dsRNA