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**Count one row of 4 squares. For cells touching the edges of the square, only count the top and left line to avoid counting the same cells twice.
**Record the number of cells (should be 30-150 cells per row) and Calculate cell concentration, where \alpha is number of cells per row
{{math|<mathvar>\&alpha\times </var> x 4 (rows per square) \times x 10^5 (dilution factor)</math>}}
[[Category:Cell Culture]]
[[Category:Knockdown]]
[[Category:Drosophila]]